The best Side of cytometer
The best Side of cytometer
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Information-enriched characterization of cell populations. Morphological details from photos can increase to your richness of flow cytometry data. Such as, the figure reveals an usually typical apoptosis assay employing Annexin V and PI, incorporating cell imaging to characterize cells in Each individual population to expose morphologically distinctive features. These insights could not are actually received from multiplex staining by itself.
Whether or not you’re a beginner or an authority, examine our methods to advance your investigation and abilities in flow cytometry.
Combine acoustic concentrating technologies with large-parameter Assessment for adaptable spectral unmixing and standard workflows.
Find new insights into your experimental knowledge by visualizing populations working with mixed fluorescence and morphology facts. Our software interface wizards permit downsampling, concatenation (merged data files) and dimensionality equipment which might be designed into the software package for an intuitive, streamlined information Assessment encounter.
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Below, a seasoned person has gated singlets confidently. Soon after evaluating the guide singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate is made up of much more than 4% aggregates.
Pair your Attune flow cytometer having an autosampler for improved workflow effectiveness, and add robotic integration for maximum throughput.
Our researchers will structure panels in your case as often times you require—cost-free, no product buy wanted.
Early indications of this contamination had in the beginning been dismissed as debris. Backgating to find new populations. Backgating imaged cells around the Attune CytPix Flow Cytometer also means that you can use morphological options to find fascinating subpopulations that could not be evident from flow cytometry knowledge alone.
People can check out occasion pictures in real-time to verify sample high-quality, improve protocols, and document the morphology of every mobile population discovered for Examination. Imaging capture also Positive aspects from acoustic concentrating, which really helps to place the cells to ensure that a sharp, centered graphic is attained.
Seem waves enable precise mobile alignment, resulting in Remarkable data high quality and ~10X a lot quicker sample flow fees.
We Formerly demonstrated the strength of imaging Motor vehicle-T/Ramos mobile interactions. Permit’s examine just the inhabitants of read more best desire, the double beneficial situations, To find out more. We can now use prolonged morphological parameters (below, circularity and skewness of intensity) to further analyze the options of those populations and refine gating on these situations, rising details robustness.
Designed-in dimensionality reduction applications greater resolution into populations. Fluorescently gated populations back again-gated onto UMAP plot to verify anticipated inhabitants resolution in lysed whole human blood. Consumer can view gatherings from Just about every cluster for visual affirmation of cell phenotypes.
No clean/no lyse leukocyte analysis with imaging. Cells have been obtained from 24-hour-outdated blood by dilution in 1 mM EDTA ( Professional UX/UI enables people to educate AI designs for expanded programs. (A) The “Educate Model” characteristic presents annotation applications and genuine-time design training accuracy.
At the center of each Attune Flow Cytometer lies our innovative acoustic focusing know-how, which utilizes seem waves to meticulously align cells throughout the flow mobile. This ground breaking tactic surpasses traditional hydrodynamic methods, leading to speedier processing speeds, Extraordinary data excellent, and greater sample throughput.
The Attune Xenith cytometer is usually a substantial-parameter spectral analyzer, providing both spectral unmixing and standard flow capabilities for complete and versatile mobile Assessment.
Agilent Worth Promise assures you no less than 10 years of instrument use from the day you buy. Usually, We are going to credit you Along with the residual price of the method toward an upgraded model.
Here, we demonstrate that by gating techniques utilizing graphic-primarily based quantitative parameters can distinguish interacting cells from coincident functions to much more accurately evaluate interacting cells.